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Immunology and Infection

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在小鼠B淋巴细胞染色体畸变所PNA-FISH快速分析

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Immunology and Infection

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JoVE Journal Immunology and Infection

Rapid Analysis of Chromosome Aberrations in Mouse B Lymphocytes by PNA-FISH

Please note that all translations are automatically generated. Click here for the English version.

在小鼠B淋巴细胞染色体畸变所PNA-FISH快速分析

DOI:

10.3791/51806-v

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07:54 min

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August 19, 2014

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Sarah M. Misenko, Samuel F. Bunting

¹Department of Molecular Biology and Biochemistry,Rutgers, the State University of New Jersey

Chapters

00:05Title
01:02B Cell Isolation and Activation
03:08B Cell Fixation, Metaphase Chromosome Spread Preparation, and Telomere PNA FISH
06:10Results: Chromosome Aberrations in Mouse B Cells as Visualized by PNA Telomere FISH
07:26Conclusion

Summary

Automatic Translation

DNA修复途径对于维持基因组的完整性，防止基因突变和癌症至关重要。该协议的目的是通过直接观察染色体畸变的量化基因组不稳定性在中期从原位杂交（FISH）对端粒DNA重复使用荧光小鼠B细胞的扩散。

Tags

Chromosome Aberrations Mouse B Lymphocytes PNA-FISH DNA Repair Genomic Instability Tumorigenesis Metaphase Chromosome Spreads Ploidy Mitotic Index Primary Cell Population Transformed Fibroblasts Patient Cell Lines

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