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A Simple and Rapid Protocol to Non-enzymatically Dissociate Fresh Human Tissues for the Analysis of Infiltrating Lymphocytes
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Immunology and Infection
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JoVE Journal Immunology and Infection
A Simple and Rapid Protocol to Non-enzymatically Dissociate Fresh Human Tissues for the Analysis of Infiltrating Lymphocytes

A Simple and Rapid Protocol to Non-enzymatically Dissociate Fresh Human Tissues for the Analysis of Infiltrating Lymphocytes

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19,785 Views

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07:29 min

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December 06, 2014

DOI:

10.3791/52392-v

DOI:
10.3791/52392-v

•

07:29 min
December 06, 2014

•

4 Views
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1Molecular Immunology Unit,Université Libre de Bruxelles, 2Institut Jules Bordet,Université Libre de Bruxelles, 3Institut d’Immunologie Médicale,Université Libre de Bruxelles, 4Flow Cytometry Core Facility,Université Libre de Bruxelles

Summary

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This protocol describes the rapid non-enzymatic dissociation of fresh human tissue fragments for qualitative and quantitative assessment of CD45+ cells (lymphocytes/leukocytes) present in various normal and malignant human tissues. Additionally, the supernatant obtained from the primary tissue homogenate can be collected and stored for further analysis or experimentation.

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Garaud, S., Gu-Trantien, C., Lodewyckx, J., Boisson, A., De Silva, P., Buisseret, L., Migliori, E., Libin, M., Naveaux, C., Duvillier, H., Willard-Gallo, K. A Simple and Rapid Protocol to Non-enzymatically Dissociate Fresh Human Tissues for the Analysis of Infiltrating Lymphocytes. J. Vis. Exp. (94), e52392, doi:10.3791/52392 (2014).

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