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Rapid Analysis of Chromosome Aberrations in Mouse B Lymphocytes by PNA-FISH
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Chapitres
- 00:05Titre
- 01:02B Cell Isolation and Activation
- 03:08B Cell Fixation, Metaphase Chromosome Spread Preparation, and Telomere PNA FISH
- 06:10Results: Chromosome Aberrations in Mouse B Cells as Visualized by PNA Telomere FISH
- 07:26Conclusion
DNA repair pathways are essential for maintenance of genomic integrity and preventing mutation and cancer. The goal of this protocol is to quantify genomic instability by direct observation of chromosome aberrations in metaphase spreads from mouse B cells using fluorescent in situ hybridization (FISH) for telomeric DNA repeats.
