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Rapid Molecular Detection and Differentiation of Influenza Viruses A and B
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Immunology and Infection
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JoVE Journal Immunology and Infection
Rapid Molecular Detection and Differentiation of Influenza Viruses A and B

Rapid Molecular Detection and Differentiation of Influenza Viruses A and B

DOI:
10.3791/54312-v

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05:38 min

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January 30, 2017

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, , , , , ,
1Department of Laboratory Medicine,Memorial Sloan Kettering Cancer Center

Chapters

  • 00:05Title
  • 00:38Before Running the Assay
  • 01:34Running the Test
  • 03:25Quality Control
  • 04:18Results: Correlation of CT Values Obtained from a Reference Method to the Results of the Influenza A and B Assay
  • 04:44Conclusion

Summary

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We describe a rapid, molecular-based Influenza A and B assay. The Influenza assay detects each target within 15 min by employing isothermal amplification with influenza-specific primers followed by target detection with molecular beacon probes. The Influenza A and B assay is user-friendly and required minimal hands-on time to perform.

Tags

Influenza VirusesRapid Molecular DetectionRespiratory InfectionsNasal SwabViral Transport MediaSample ReceiverTest BaseInstrumentPatient IDSample ProcessingTransfer Cartridge

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