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재조합 셀룰라아제 Cel5A의 표현에서<em> 트리코더마 reesei</em> 담배 식물에서
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Expression of Recombinant Cellulase Cel5A from Trichoderma reesei in Tobacco Plants
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재조합 셀룰라아제 Cel5A의 표현에서<em> 트리코더마 reesei</em> 담배 식물에서

English

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12,681 Views

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13:05 min

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June 13, 2014

DOI:

10.3791/51711-v

DOI:
10.3791/51711-v

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13:05 min
June 13, 2014

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7 Views
, , , ,
1Institute for Molecular Biotechnology, Bio7,RWTH Aachen University, 2Fraunhofer Institute for Molecular Biology and Applied Ecology

Summary

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담배 식물은 일시적 발현 시스템을 통해, 진균 셀룰라아제, TrCel5A을 제조 하였다. 발현은 형광 융합 단백질을 사용하여 모니터링 할 수 있고, 단백질 활성은 사후 발현 분석되었다.

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Cite this Article

Garvey, M., Klinger, J., Klose, H., Fischer, R., Commandeur, U. Expression of Recombinant Cellulase Cel5A from Trichoderma reesei in Tobacco Plants. J. Vis. Exp. (88), e51711, doi:10.3791/51711 (2014).

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