Journal
/
Ambiente
/
Expression of Recombinant Cellulase Cel5A from Trichoderma reesei in Tobacco Plants
JoVE Journal
Ambiente
É necessária uma assinatura da JoVE para visualizar este conteúdo.  Faça login ou comece sua avaliação gratuita.
JoVE Journal Ambiente
Expression of Recombinant Cellulase Cel5A from Trichoderma reesei in Tobacco Plants

Expression of Recombinant Cellulase Cel5A from Trichoderma reesei in Tobacco Plants

12,687 Views

•

13:05 min

•

June 13, 2014

DOI:

10.3791/51711-v

DOI:
10.3791/51711-v

•

13:05 min
June 13, 2014

•

7 Views
, , , ,
1Institute for Molecular Biotechnology, Bio7,RWTH Aachen University, 2Fraunhofer Institute for Molecular Biology and Applied Ecology

Summary

Automatically generated

Tobacco plants were used to produce a fungal cellulase, TrCel5A, via a transient expression system. The expression could be monitored using a fluorescent fusion protein, and the protein activity was characterized post-expression.

Explore More Videos

CellulaseTrichoderma ReeseiCel5ARecombinant ExpressionTobacco PlantsTransient ExpressionEndoglucanaseSDS-PAGEWestern BlotZymographySubstrate Degradation Assay

Read Article

Cite this Article

Garvey, M., Klinger, J., Klose, H., Fischer, R., Commandeur, U. Expression of Recombinant Cellulase Cel5A from Trichoderma reesei in Tobacco Plants. J. Vis. Exp. (88), e51711, doi:10.3791/51711 (2014).

Download .ris file

Copy

Share Video

.

Copy