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Expression of Recombinant Cellulase Cel5A from Trichoderma reesei in Tobacco Plants
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JoVE Journal Çevre
Expression of Recombinant Cellulase Cel5A from Trichoderma reesei in Tobacco Plants

Expression of Recombinant Cellulase Cel5A from Trichoderma reesei in Tobacco Plants

DOI:
10.3791/51711-v

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13:05 min

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June 13, 2014

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, , , ,
1Institute for Molecular Biotechnology, Bio7,RWTH Aachen University, 2Fraunhofer Institute for Molecular Biology and Applied Ecology

Bölümler

  • 00:05Başlık
  • 01:32Transient Expression of TrCel5A and TrCel5A-mCherry Proteins
  • 03:17TrCel5A Extraction from Tobacco Leaves
  • 04:42SDS-PAGE, Western Blotting, and Azo-CMC Zymography of TrCel5A-mCherry
  • 06:394-MUC Activity Assay of TrCel5A
  • 07:50Azo-carboxymethyl Cellulose Activity Assay of TrCel5A
  • 09:01PAHBAH Assay of TrCel5A
  • 10:40Results: Expression of Recombinant Cellulase Cel5A from Trichoderma reesei in Tobacco Plants
  • 12:24Conclusion

Özet

Otomatik Çeviri

Otomatik Çeviri

Tobacco plants were used to produce a fungal cellulase, TrCel5A, via a transient expression system. The expression could be monitored using a fluorescent fusion protein, and the protein activity was characterized post-expression.

Etiketler

CellulaseTrichoderma ReeseiCel5ARecombinant ExpressionTobacco PlantsTransient ExpressionEndoglucanaseSDS-PAGEWestern BlotZymographySubstrate Degradation Assay

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