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Expression of Recombinant Cellulase Cel5A from Trichoderma reesei in Tobacco Plants
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Expression of Recombinant Cellulase Cel5A from Trichoderma reesei in Tobacco Plants

Expression of Recombinant Cellulase Cel5A from Trichoderma reesei in Tobacco Plants

12,687 Views

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13:05 min

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June 13, 2014

DOI:

10.3791/51711-v

DOI:
10.3791/51711-v

•

13:05 min
June 13, 2014

•

7 Views
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1Institute for Molecular Biotechnology, Bio7,RWTH Aachen University, 2Fraunhofer Institute for Molecular Biology and Applied Ecology

Summary

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Tobacco plants were used to produce a fungal cellulase, TrCel5A, via a transient expression system. The expression could be monitored using a fluorescent fusion protein, and the protein activity was characterized post-expression.

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CellulaseTrichoderma ReeseiCel5ARecombinant ExpressionTobacco PlantsTransient ExpressionEndoglucanaseSDS-PAGEWestern BlotZymographySubstrate Degradation Assay

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Cite this Article

Garvey, M., Klinger, J., Klose, H., Fischer, R., Commandeur, U. Expression of Recombinant Cellulase Cel5A from Trichoderma reesei in Tobacco Plants. J. Vis. Exp. (88), e51711, doi:10.3791/51711 (2014).

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