Journal
/
Environment
/
組換えセルラーゼCEL5Aの式から<em>トリコデルマ·</em>タバコ植物における
JoVE Journal
Environment
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Environment
Expression of Recombinant Cellulase Cel5A from Trichoderma reesei in Tobacco Plants
Please note that all translations are automatically generated. Click here for the English version.

組換えセルラーゼCEL5Aの式から<em>トリコデルマ·</em>タバコ植物における

日本語

Automatically Generated

12,687 Views

•

13:05 min

•

June 13, 2014

DOI:

10.3791/51711-v

DOI:
10.3791/51711-v

•

13:05 min
June 13, 2014

•

7 Views
, , , ,
1Institute for Molecular Biotechnology, Bio7,RWTH Aachen University, 2Fraunhofer Institute for Molecular Biology and Applied Ecology

Summary

Automatically generated

タバコ植物を、一過性発現系を介して、真菌セルラーゼ、TrCel5Aを作製した。発現は、蛍光融合タンパク質を用いてモニターすることができ、タンパク質活性を発現後に特徴付けた。

Explore More Videos

CellulaseTrichoderma ReeseiCel5ARecombinant ExpressionTobacco PlantsTransient ExpressionEndoglucanaseSDS-PAGEWestern BlotZymographySubstrate Degradation Assay

Read Article

Cite this Article

Garvey, M., Klinger, J., Klose, H., Fischer, R., Commandeur, U. Expression of Recombinant Cellulase Cel5A from Trichoderma reesei in Tobacco Plants. J. Vis. Exp. (88), e51711, doi:10.3791/51711 (2014).

Download .ris file

Copy

Share Video

.

Copy