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High Yield Zuivering van<em> Plasmodium falciparum</em> Merozoïeten Voor gebruik in opsoniserende antilichaamassays
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
High Yield Purification of Plasmodium falciparum Merozoites For Use in Opsonizing Antibody Assays
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High Yield Zuivering van<em> Plasmodium falciparum</em> Merozoïeten Voor gebruik in opsoniserende antilichaamassays

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22,524 Views

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10:38 min

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July 17, 2014

DOI:

10.3791/51590-v

DOI:
10.3791/51590-v

•

10:38 min
July 17, 2014

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4 Views
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1Division of Infection and Immunity,Walter and Eliza Hall Institute of Medical Research, 2Department of Medical Biology,University of Melbourne

Summary

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Het meten van antilichaam-functie is de sleutel tot het begrijpen van immuniteit tegen Plasmodium falciparum malaria. Deze methode beschrijft de zuivering van levensvatbare merozoïeten en meting van opsonisatie-afhankelijke fagocytose door flowcytometrie.

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Plasmodium FalciparumMerozoitesMalaria VaccineOpsonizing AntibodiesPhagocytosisTHP-1 CellsE64 Protease InhibitorHigh-yield PurificationAssay Development

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Hill, D. L., Eriksson, E. M., Schofield, L. High Yield Purification of Plasmodium falciparum Merozoites For Use in Opsonizing Antibody Assays. J. Vis. Exp. (89), e51590, doi:10.3791/51590 (2014).

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