Journal
/
Inmunología y contagio
/
High Yield Purification of Plasmodium falciparum Merozoites For Use in Opsonizing Antibody Assays
JoVE Revista
Inmunología y contagio
This content is Free Access.
JoVE Revista Inmunología y contagio
High Yield Purification of Plasmodium falciparum Merozoites For Use in Opsonizing Antibody Assays

High Yield Purification of Plasmodium falciparum Merozoites For Use in Opsonizing Antibody Assays

22,527 Views

•

10:38 min

•

July 17, 2014

DOI:

10.3791/51590-v

DOI:
10.3791/51590-v

•

10:38 min
July 17, 2014

•

4 Views
, ,
1Division of Infection and Immunity,Walter and Eliza Hall Institute of Medical Research, 2Department of Medical Biology,University of Melbourne

Summary

Automatically generated

Measuring antibody function is key to understanding immunity to Plasmodium falciparum malaria. This method describes the purification of viable merozoites, and measurement of opsonization-dependent phagocytosis by flow cytometry.

Explore More Videos

Plasmodium FalciparumMerozoitesMalaria VaccineOpsonizing AntibodiesPhagocytosisTHP-1 CellsE64 Protease InhibitorHigh-yield PurificationAssay Development

Read Article

Cite this Article

Hill, D. L., Eriksson, E. M., Schofield, L. High Yield Purification of Plasmodium falciparum Merozoites For Use in Opsonizing Antibody Assays. J. Vis. Exp. (89), e51590, doi:10.3791/51590 (2014).

Download .ris file

Copy

Share Video

.

Copy