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High Yield Purification of Plasmodium falciparum Merozoites For Use in Opsonizing Antibody Assays
JoVE 杂志
免疫与感染
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JoVE 杂志 免疫与感染
High Yield Purification of Plasmodium falciparum Merozoites For Use in Opsonizing Antibody Assays

High Yield Purification of Plasmodium falciparum Merozoites For Use in Opsonizing Antibody Assays

DOI:
10.3791/51590-v

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10:38 min

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July 17, 2014

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, ,
1Division of Infection and Immunity,Walter and Eliza Hall Institute of Medical Research, 2Department of Medical Biology,University of Melbourne

Chapters

  • 00:05Title
  • 01:34Isolation of P. falciparum Merozoites
  • 04:42Quantifying Merozoite Concentration by Flow Cytometry
  • 06:03Phagocytosis Assay
  • 07:48Results: The Merzoite:THP-1 Ratio Influences the Degree of Phagocytosis Observed
  • 09:38Conclusion

Summary

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Measuring antibody function is key to understanding immunity to Plasmodium falciparum malaria. This method describes the purification of viable merozoites, and measurement of opsonization-dependent phagocytosis by flow cytometry.

Tags

Plasmodium FalciparumMerozoitesMalaria VaccineOpsonizing AntibodiesPhagocytosisTHP-1 CellsE64 Protease InhibitorHigh-yield PurificationAssay Development

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