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High Yield Purification of Plasmodium falciparum Merozoites For Use in Opsonizing Antibody Assays
JoVE Journal
İmmünoloji ve Enfeksiyon
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JoVE Journal İmmünoloji ve Enfeksiyon
High Yield Purification of Plasmodium falciparum Merozoites For Use in Opsonizing Antibody Assays

High Yield Purification of Plasmodium falciparum Merozoites For Use in Opsonizing Antibody Assays

22,527 Views

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10:38 min

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July 17, 2014

DOI:

10.3791/51590-v

DOI:
10.3791/51590-v

•

10:38 min
July 17, 2014

•

4 Views
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1Division of Infection and Immunity,Walter and Eliza Hall Institute of Medical Research, 2Department of Medical Biology,University of Melbourne

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Measuring antibody function is key to understanding immunity to Plasmodium falciparum malaria. This method describes the purification of viable merozoites, and measurement of opsonization-dependent phagocytosis by flow cytometry.

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Plasmodium FalciparumMerozoitesMalaria VaccineOpsonizing AntibodiesPhagocytosisTHP-1 CellsE64 Protease InhibitorHigh-yield PurificationAssay Development

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Hill, D. L., Eriksson, E. M., Schofield, L. High Yield Purification of Plasmodium falciparum Merozoites For Use in Opsonizing Antibody Assays. J. Vis. Exp. (89), e51590, doi:10.3791/51590 (2014).

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