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人类单核双梯度离心法和其分化为巨噬细胞的特富龙涂层的细胞培养袋隔离
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Isolation of Human Monocytes by Double Gradient Centrifugation and Their Differentiation to Macrophages in Teflon-coated Cell Culture Bags
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人类单核双梯度离心法和其分化为巨噬细胞的特富龙涂层的细胞培养袋隔离

DOI:
10.3791/51554-v

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09:32 min

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September 09, 2014

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, , , , , ,
1Department of Hematology and Oncology,University Medical Center Göttingen, 2Microbial Interface Biology Group,Research Center Borstel

Chapters

  • 00:05Title
  • 01:27Isolating Monocytes
  • 04:29Differentiation of Monocytes to Macrophages
  • 05:39Macrophage Harvest
  • 07:09Macrophage Yields and Characterization
  • 08:52Conclusion

Summary

Automatic Translation

Automatic Translation

我们提出了一个简单而有效的协议对人类巨噬细胞的产生。血沉棕黄层用双密度梯度离心法处理，并分离单核细胞，然后分化为巨噬细胞中的特氟龙涂层的细胞培养袋。这最大限度地提高巨噬细胞的产量，并促进细胞收集在后续实验。

Tags

Human MonocytesDouble Gradient CentrifugationMacrophage DifferentiationTeflon-coated Cell Culture BagsM-CSFMacrophage IsolationQuality ControlSyngeneic Human System

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