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薄层色谱(TLC)分离和植物提取物的生物测定识别抗菌化合物
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Thin-layer Chromatographic (TLC) Separations and Bioassays of Plant Extracts to Identify Antimicrobial Compounds
Please note that all translations are automatically generated.
Click here for the English version.
薄层色谱(TLC)分离和植物提取物的生物测定识别抗菌化合物
DOI:
10.3791/51411-v
•
12:04 min
•
March 27, 2014
•
Isabelle A. Kagan
,
Michael D. Flythe
1
Forage-Animal Production Research Unit, Agricultural Research Service
,
United States Department of Agriculture
Chapters
00:05
Title
01:22
Preparation of Thin-layer Plates
03:39
Preparation of Developing Chambers for Extract Separation
04:23
Loading and Development of TLC Plates
06:04
Preparation of Plates for Bioassay
07:41
Bacterial Culture and Assay
09:14
Visualization of Bioassayed Agar Plates
10:07
TLC and Contact Bioautography Assay Results
11:21
Conclusion
Summary
Automatic Translation
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Automatic Translation
方法描述为薄层色谱(TLC)分离植物提取物和生物自显影接触,以确定抗菌代谢产物。该方法适用于红三叶草酚类化合物抑制超氨生产菌(HAB),原产于牛瘤胃的筛选。
Tags
Thin-layer Chromatography
TLC
Bioassay
Plant Extract
Antimicrobial Compounds
Bioautography
Microbial Growth
UV Visualization
Tetrazolium Red Staining
Clostridium Sticklandii
Red Clover
Phenolic Compounds
Hyper Ammonia-producing Bacteria
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