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Thin-layer Chromatographic (TLC) Separations and Bioassays of Plant Extracts to Identify Antimicrobial Compounds
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Chemistry
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JoVE Journal Chemistry
Thin-layer Chromatographic (TLC) Separations and Bioassays of Plant Extracts to Identify Antimicrobial Compounds

Thin-layer Chromatographic (TLC) Separations and Bioassays of Plant Extracts to Identify Antimicrobial Compounds

English

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66,637 Views

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12:04 min

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March 27, 2014

DOI:

10.3791/51411-v

DOI:
10.3791/51411-v

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12:04 min
March 27, 2014

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39 Views
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1Forage-Animal Production Research Unit, Agricultural Research Service,United States Department of Agriculture

Summary

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Methods are described for thin-layer chromatographic (TLC) separation of plant extracts and contact bioautography to identify antibacterial metabolites. The methods are applied to the screening of red clover phenolic compounds inhibiting hyper ammonia-producing bacteria (HAB) native to the bovine rumen.

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Kagan, I. A., Flythe, M. D. Thin-layer Chromatographic (TLC) Separations and Bioassays of Plant Extracts to Identify Antimicrobial Compounds. J. Vis. Exp. (85), e51411, doi:10.3791/51411 (2014).

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