Journal
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Immunology and Infection
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Rapid Screening of HIV Reverse Transcriptase and Integrase Inhibitors
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Rapid Screening of HIV Reverse Transcriptase and Integrase Inhibitors

Rapid Screening of HIV Reverse Transcriptase and Integrase Inhibitors

English

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17,463 Views

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05:46 min

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April 09, 2014

DOI:

10.3791/51400-v

DOI:
10.3791/51400-v

•

05:46 min
April 09, 2014

•

9 Views
,
1HIV Drug Resistance Program,National Cancer Institute

Summary

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Here we describe cellular cytotoxicity and single round infectivity assays that allow for the rapid and accurate screening of compounds to determine their cellular cytotoxicity (CC50) and IC50 values against WT and drug resistant HIV-1.

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HIV Reverse TranscriptaseHIV IntegraseAntiviral CompoundsDrug ResistanceCytotoxicity AssayLuciferase ReporterViral InfectivityScreening PlatformHigh-throughput Screening

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Cite this Article

Smith, S. J., Hughes, S. H. Rapid Screening of HIV Reverse Transcriptase and Integrase Inhibitors. J. Vis. Exp. (86), e51400, doi:10.3791/51400 (2014).

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