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Rapid Screening of HIV Reverse Transcriptase and Integrase Inhibitors
JoVE Journal
İmmünoloji ve Enfeksiyon
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JoVE Journal İmmünoloji ve Enfeksiyon
Rapid Screening of HIV Reverse Transcriptase and Integrase Inhibitors

Rapid Screening of HIV Reverse Transcriptase and Integrase Inhibitors

DOI:
10.3791/51400-v

•

05:46 min

•

April 09, 2014

•

,
1HIV Drug Resistance Program,National Cancer Institute

Bölümler

  • 00:05Başlık
  • 01:09Viral Stock Generation
  • 02:29Compound Screening
  • 03:42Measurement of Luciferase Activity
  • 04:41Results: Representative Luciferase Signal Readout Inhibition
  • 05:22Conclusion

Özet

Otomatik Çeviri

Otomatik Çeviri

Here we describe cellular cytotoxicity and single round infectivity assays that allow for the rapid and accurate screening of compounds to determine their cellular cytotoxicity (CC50) and IC50 values against WT and drug resistant HIV-1.

Etiketler

HIV Reverse TranscriptaseHIV IntegraseAntiviral CompoundsDrug ResistanceCytotoxicity AssayLuciferase ReporterViral InfectivityScreening PlatformHigh-throughput Screening

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