Journal
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Inmunología y contagio
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Rapid Screening of HIV Reverse Transcriptase and Integrase Inhibitors
JoVE Revista
Inmunología y contagio
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JoVE Revista Inmunología y contagio
Rapid Screening of HIV Reverse Transcriptase and Integrase Inhibitors

Rapid Screening of HIV Reverse Transcriptase and Integrase Inhibitors

DOI:
10.3791/51400-v

•

05:46 min

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April 09, 2014

•

,
1HIV Drug Resistance Program,National Cancer Institute

Capítulos

  • 00:05Título
  • 01:09Viral Stock Generation
  • 02:29Compound Screening
  • 03:42Measurement of Luciferase Activity
  • 04:41Results: Representative Luciferase Signal Readout Inhibition
  • 05:22Conclusion

Summary

Traducción Automática

Traducción Automática

Here we describe cellular cytotoxicity and single round infectivity assays that allow for the rapid and accurate screening of compounds to determine their cellular cytotoxicity (CC50) and IC50 values against WT and drug resistant HIV-1.

Tags

HIV Reverse TranscriptaseHIV IntegraseAntiviral CompoundsDrug ResistanceCytotoxicity AssayLuciferase ReporterViral InfectivityScreening PlatformHigh-throughput Screening

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