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08:46 min
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January 15, 2014
DOI:
1Department of Biomedical and Molecular Sciences,Queen’s University, 2National Institute of Neurological Disorders and Stroke,Porter Neuroscience Research Center, 3Research Institute of Genome-Based Biofactory,National Institute of Advanced Industrial Science and Technology, 4The Robert H. Smith Faculty of Agriculture, Food and Environment, Institute of Biochemistry, Food Science, and Nutrition,The Hebrew University of Jerusalem
Summary
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Le proteine antigelo (AFP) si legano a specifici piani di ghiaccio per prevenire o rallentare la crescita del ghiaccio. L'analisi FIPA (Ice Plane Affinity) basata sulla fluorescenza è una modifica del metodo originale di incisione del ghiaccio per la determinazione dei piani di ghiaccio legati all'AFP. Gli ARP sono etichettati fluorescentmente, incorporati in cristalli di ghiaccio singoli macroscopici e visualizzati sotto la luce UV.
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Cite this Article
Basu, K., Garnham, C. P., Nishimiya, Y., Tsuda, S., Braslavsky, I., Davies, P. Determining the Ice-binding Planes of Antifreeze Proteins by Fluorescence-based Ice Plane Affinity. J. Vis. Exp. (83), e51185, doi:10.3791/51185 (2014).
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