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通过荧光基冰平面亲和力确定防冻蛋白的冰结合平面
JoVE Journal
Chemistry
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JoVE Journal Chemistry
Determining the Ice-binding Planes of Antifreeze Proteins by Fluorescence-based Ice Plane Affinity
DOI:

08:46 min

January 15, 2014

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Chapters

  • 00:05Title
  • 01:14Growing Single Ice Crystals
  • 03:18Determining Singularity and Orientation o the Ice Crystal
  • 04:37Absorption of Fluorescently Labeled Antifreeze Proteins to a Single Ice Crystal
  • 07:20Results: Fluorescence-based Ice Plane Affinity Analysis Images
  • 08:18Conclusion

Summary

Automatic Translation

防冻蛋白 (AFP) 与特定的冰平面结合,以防止或减缓冰的生长。基于荧光的冰平面亲和力 (FIPA) 分析是对确定 AFP 绑定冰平面的原始冰蚀刻方法的修改。AFP 采用荧光标记,并融入宏观单一冰晶中,并在紫外线下可视化。

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