A subscription to JoVE is required to view this content. Sign in or start your free trial.
1Department of Biomedical and Molecular Sciences,Queen’s University, 2National Institute of Neurological Disorders and Stroke,Porter Neuroscience Research Center, 3Research Institute of Genome-Based Biofactory,National Institute of Advanced Industrial Science and Technology, 4The Robert H. Smith Faculty of Agriculture, Food and Environment, Institute of Biochemistry, Food Science, and Nutrition,The Hebrew University of Jerusalem
Chapters
- 00:05Title
- 01:14Growing Single Ice Crystals
- 03:18Determining Singularity and Orientation o the Ice Crystal
- 04:37Absorption of Fluorescently Labeled Antifreeze Proteins to a Single Ice Crystal
- 07:20Results: Fluorescence-based Ice Plane Affinity Analysis Images
- 08:18Conclusion
Frostschutzproteine (AFPs) binden an bestimmte Eisebenen, um das Eiswachstum zu verhindern oder zu verlangsamen. Die Fluoreszenzbasierte Eisebene (FIPA)-Analyse ist eine Modifikation der ursprünglichen Eisätzmethode zur Bestimmung von AFP-gebundenen Eisebenen. AFPs werden fluoreszierend beschriftet, in makroskopische Einzeleiskristalle integriert und unter UV-Licht visualisiert.
