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Methodik zur effizienten Erzeugung fluoreszierender Vaccinia-Virusproteine
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Methodology for the Efficient Generation of Fluorescently Tagged Vaccinia Virus Proteins
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Methodik zur effizienten Erzeugung fluoreszierender Vaccinia-Virusproteine

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7,262 Views

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09:27 min

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January 17, 2014

DOI:

10.3791/51151-v

DOI:
10.3791/51151-v

•

09:27 min
January 17, 2014

•

2 Views
, , , , ,
1School of Molecular Bioscience,University of Sydney, 2Department of Medicine,Center for Vascular Research, 3Asia Pacific Centre for Animal Health, Faculty of Veterinary Science,University of Melbourne

Summary

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Hier wird ein schnelles und modulares Protokoll zur Erzeugung rekombinanter Impfstoffviren beschrieben, die fluoreszierend markierte Proteine gleichzeitig mit der Methode der transienten dominanten Selektion exezieren.

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Cite this Article

Marzook, N. B., Procter, D. J., Lynn, H., Yamamoto, Y., Horsington, J., Newsome, T. P. Methodology for the Efficient Generation of Fluorescently Tagged Vaccinia Virus Proteins. J. Vis. Exp. (83), e51151, doi:10.3791/51151 (2014).

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