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Journal
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Immunology and Infection
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从新型H7N9流感病毒利用杆状病毒表达系统的功能重组血凝素和神经氨酸酶蛋白的表达
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Immunology and Infection
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JoVE Journal
Immunology and Infection
Expression of Functional Recombinant Hemagglutinin and Neuraminidase Proteins from the Novel H7N9 Influenza Virus Using the Baculovirus Expression System
Please note that all translations are automatically generated.
Click here for the English version.
从新型H7N9流感病毒利用杆状病毒表达系统的功能重组血凝素和神经氨酸酶蛋白的表达
DOI:
10.3791/51112-v
•
12:18 min
•
November 06, 2013
•
Irina Margine
2
,
Peter Palese
3
,
Florian Krammer
1
Department of Microbiology
,
Icahn School of Medicine at Mount Sinai
,
2
Graduate School of Biological Sciences
,
Icahn School of Medicine at Mount Sinai
,
3
Department of Medicine
,
Icahn School of Medicine at Mount Sinai
Chapters
00:05
Title
01:44
Rescue of Recombinant Baculovirus and Verification of Protein Expression
04:13
Protein Expression
06:15
Protein Purification
09:52
Results: Quality Control of the Harvested Proteins
11:49
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
在这里,我们描述来表达在昆虫细胞来源于小说中国H7N9病毒的正确折叠和功能的流感病毒表面抗原的方法。该技术可适于表达任何病毒或细胞表面蛋白的胞外结构域。
Tags
Baculovirus Expression System
Recombinant Hemagglutinin
Recombinant Neuraminidase
H7N9 Influenza Virus
Glycoproteins
Immunological Assays
Vaccine Standardization
Antibody Discovery
NA Enzymatic Function
Antivirals
Recombinant Vaccine
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