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Expression of Functional Recombinant Hemagglutinin and Neuraminidase Proteins from the Novel H7N9 Influenza Virus Using the Baculovirus Expression System
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Immunologia e infezioni
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JoVE Journal Immunologia e infezioni
Expression of Functional Recombinant Hemagglutinin and Neuraminidase Proteins from the Novel H7N9 Influenza Virus Using the Baculovirus Expression System

Expression of Functional Recombinant Hemagglutinin and Neuraminidase Proteins from the Novel H7N9 Influenza Virus Using the Baculovirus Expression System

DOI:
10.3791/51112-v

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12:18 min

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November 06, 2013

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, ,
1Department of Microbiology,Icahn School of Medicine at Mount Sinai, 2Graduate School of Biological Sciences,Icahn School of Medicine at Mount Sinai, 3Department of Medicine,Icahn School of Medicine at Mount Sinai

Capitoli

  • 00:05Titolo
  • 01:44Rescue of Recombinant Baculovirus and Verification of Protein Expression
  • 04:13Protein Expression
  • 06:15Protein Purification
  • 09:52Results: Quality Control of the Harvested Proteins
  • 11:49Conclusion

Summary

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Here we describe a way to express correctly folded and functional influenza virus surface antigens derived from the novel Chinese H7N9 virus in insect cells. The technique can be adapted to express ectodomains of any viral or cellular surface proteins.

Tags

Baculovirus Expression SystemRecombinant HemagglutininRecombinant NeuraminidaseH7N9 Influenza VirusGlycoproteinsImmunological AssaysVaccine StandardizationAntibody DiscoveryNA Enzymatic FunctionAntiviralsRecombinant Vaccine

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