Journal
/
Immunology and Infection
/
Expression of Functional Recombinant Hemagglutinin and Neuraminidase Proteins from the Novel H7N9 Influenza Virus Using the Baculovirus Expression System
JoVE Journal
Immunology and Infection
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Immunology and Infection
Expression of Functional Recombinant Hemagglutinin and Neuraminidase Proteins from the Novel H7N9 Influenza Virus Using the Baculovirus Expression System

Expression of Functional Recombinant Hemagglutinin and Neuraminidase Proteins from the Novel H7N9 Influenza Virus Using the Baculovirus Expression System

DOI:
10.3791/51112-v

•

12:18 min

•

November 06, 2013

•

, ,
1Department of Microbiology,Icahn School of Medicine at Mount Sinai, 2Graduate School of Biological Sciences,Icahn School of Medicine at Mount Sinai, 3Department of Medicine,Icahn School of Medicine at Mount Sinai

Chapters

  • 00:05Title
  • 01:44Rescue of Recombinant Baculovirus and Verification of Protein Expression
  • 04:13Protein Expression
  • 06:15Protein Purification
  • 09:52Results: Quality Control of the Harvested Proteins
  • 11:49Conclusion

Summary

Automatic Translation

Automatic Translation

Here we describe a way to express correctly folded and functional influenza virus surface antigens derived from the novel Chinese H7N9 virus in insect cells. The technique can be adapted to express ectodomains of any viral or cellular surface proteins.

Tags

Baculovirus Expression SystemRecombinant HemagglutininRecombinant NeuraminidaseH7N9 Influenza VirusGlycoproteinsImmunological AssaysVaccine StandardizationAntibody DiscoveryNA Enzymatic FunctionAntiviralsRecombinant Vaccine

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

Generation of Recombinant Influenza Virus from Plasmid DNA

Using Bioluminescent Imaging to Investigate Synergism Between Streptococcus pneumoniae and Influenza A Virus in Infant Mice

Packaging HIV- or FIV-based Lentivector Expression Constructs & Transduction of VSV-G Pseudotyped Viral Particles

Affinity Purification of Influenza Virus Ribonucleoprotein Complexes from the Chromatin of Infected Cells

Differentiating Functional Roles of Gene Expression from Immune and Non-immune Cells in Mouse Colitis by Bone Marrow Transplantation

Rescue of Recombinant Newcastle Disease Virus from cDNA

Scalable High Throughput Selection From Phage-displayed Synthetic Antibody Libraries

Co-immunoprecipitation of the Mouse Mx1 Protein with the Influenza A Virus Nucleoprotein

Nasal Wipes for Influenza A Virus Detection and Isolation from Swine

Fluorescence-based Neuraminidase Inhibition Assay to Assess the Susceptibility of Influenza Viruses to The Neuraminidase Inhibitor Class of Antivirals

Read Article