/
/
Cecal ligadura e punção induzida Sepsis como um modelo para estudar a autofagia em Ratos
A subscription to JoVE is required to view this content. Sign in or start your free trial.
1Department of Medicine, Division of Pulmonary and Critical Care Medicine,Brigham and Women’s Hospital, 2Department of Medicine, Renal Division,Brigham and Women’s Hospital, Harvard Medical School, 3First Department of Critical Care Medicine and Pulmonary Services,University of Athens Medical School, Evangelismos Hospital, Athens, Greece
Chapters
- 00:05Title
- 01:45Cecal Ligation and Puncture
- 03:44Tissue harvest and Fixation
- 05:06Results: Autophagy in Mice after Cecal Ligation and Puncture
- 06:05Conclusion
Sepse experimental pode ser induzida em camundongos usando a ligadura e punção do ceco método (CLP). Protocolos correntes para avaliar a autofagia in vivo no contexto de sepsia induzida por CLP são aqui apresentados: Um protocolo para medir autofagia utilizando ratinhos (GFP)-LC3, e um protocolo para medir a formação de autophagosome por microscopia electrónica.
