/
/
Cecal ligadura e punção induzida Sepsis como um modelo para estudar a autofagia em Ratos
A subscription to JoVE is required to view this content. Sign in or start your free trial.
27,978 Views
•
06:40 min
•
February 09, 2014
DOI:
1Department of Medicine, Division of Pulmonary and Critical Care Medicine,Brigham and Women’s Hospital, 2Department of Medicine, Renal Division,Brigham and Women’s Hospital, Harvard Medical School, 3First Department of Critical Care Medicine and Pulmonary Services,University of Athens Medical School, Evangelismos Hospital, Athens, Greece
Summary
Automatically generated
Sepse experimental pode ser induzida em camundongos usando a ligadura e punção do ceco método (CLP). Protocolos correntes para avaliar a autofagia in vivo no contexto de sepsia induzida por CLP são aqui apresentados: Um protocolo para medir autofagia utilizando ratinhos (GFP)-LC3, e um protocolo para medir a formação de autophagosome por microscopia electrónica.
Read Article
Cite this Article
Siempos, I. I., Lam, H. C., Ding, Y., Choi, M. E., Choi, A. M. K., Ryter, S. W. Cecal Ligation and Puncture-induced Sepsis as a Model To Study Autophagy in Mice. J. Vis. Exp. (84), e51066, doi:10.3791/51066 (2014).
Copy