/
/
Cecal Ligation and Puncture-induced Sepsis as a Model To Study Autophagy in Mice
A subscription to JoVE is required to view this content. Sign in or start your free trial.
27,978 Views
•
06:40 min
•
February 09, 2014
DOI:
1Department of Medicine, Division of Pulmonary and Critical Care Medicine,Brigham and Women’s Hospital, 2Department of Medicine, Renal Division,Brigham and Women’s Hospital, Harvard Medical School, 3First Department of Critical Care Medicine and Pulmonary Services,University of Athens Medical School, Evangelismos Hospital, Athens, Greece
Summary
Automatically generated
Experimental sepsis can be induced in mice using the cecal ligation and puncture (CLP) method. Current protocols to assess autophagy in vivo in the context of CLP-induced sepsis are presented here: A protocol for measuring autophagy using (GFP)-LC3 mice, and a protocol for measuring autophagosome formation by electron microscopy.
Read Article
Cite this Article
Siempos, I. I., Lam, H. C., Ding, Y., Choi, M. E., Choi, A. M. K., Ryter, S. W. Cecal Ligation and Puncture-induced Sepsis as a Model To Study Autophagy in Mice. J. Vis. Exp. (84), e51066, doi:10.3791/51066 (2014).
Copy