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Immunology and Infection

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JoVE Journal Immunology and Infection
Cecal Ligation and Puncture-induced Sepsis as a Model To Study Autophagy in Mice
 

Cecal Ligation and Puncture-induced Sepsis as a Model To Study Autophagy in Mice

Article DOI: 10.3791/51066-v • 06:40 min • February 9th, 2014
February 9th, 2014 •
1,3, 1, 2, 2, 1, 1
1Department of Medicine, Division of Pulmonary and Critical Care Medicine, Brigham and Women's Hospital, 2Department of Medicine, Renal Division, Brigham and Women's Hospital, Harvard Medical School, 3First Department of Critical Care Medicine and Pulmonary Services, University of Athens Medical School, Evangelismos Hospital, Athens, Greece

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Resumen
Automatic Translation

Experimental sepsis can be induced in mice using the cecal ligation and puncture (CLP) method. Current protocols to assess autophagy in vivo in the context of CLP-induced sepsis are presented here: A protocol for measuring autophagy using (GFP)-LC3 mice, and a protocol for measuring autophagosome formation by electron microscopy.

Tags

Cecal Ligation And Puncture CLP-induced Sepsis Experimental Sepsis Autophagy In Mice GFP-LC3 Fusion Protein Enhanced Autophagosome Formation Activation Of The Autophagy Pathway Autophagic Vacuole Electron Microscopy Understanding Sepsis Pathogenesis Critical Care Medicine
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