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Nucleofection von Nagerneuroblasten zu Neuroblast Migrationsstudie<em> In-vitro-</em
JoVE Journal
Neuroscience
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JoVE Journal Neuroscience
Nucleofection of Rodent Neuroblasts to Study Neuroblast Migration In vitro
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Nucleofection von Nagerneuroblasten zu Neuroblast Migrationsstudie<em> In-vitro-</em

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11,244 Views

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11:32 min

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November 12, 2013

DOI:

10.3791/50989-v

DOI:
10.3791/50989-v

•

11:32 min
November 12, 2013

•

1 Views
, , , ,
1Wolfson Centre for Age-Related Diseases,King’s College London, 2MRC Centre for Developmental Neurobiology,King’s College London

Summary

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Neuroblast Migration ist ein entscheidender Schritt in der postnatalen Neurogenese. Das hier beschriebene Protokoll kann verwendet werden, um die Rolle der Regulatoren der Neuroblast Kandidat Migration durch den Einsatz von DNA / kleine Haarnadel-RNA (shRNA) Nukleofektion und eine 3D-Migrationsassay mit Neuroblasten aus dem Nagetier postnatale rostralen Migrationsstrom getrennt zu untersuchen.

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Cite this Article

Falenta, K., Gajendra, S., Sonego, M., Doherty, P., Lalli, G. Nucleofection of Rodent Neuroblasts to Study Neuroblast Migration In vitro. J. Vis. Exp. (81), e50989, doi:10.3791/50989 (2013).

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