ジャーナル
/
神経科学
/
Nucleofection of Rodent Neuroblasts to Study Neuroblast Migration In vitro
JoVE Journal
神経科学
This content is Free Access.
JoVE Journal 神経科学
Nucleofection of Rodent Neuroblasts to Study Neuroblast Migration In vitro

Nucleofection of Rodent Neuroblasts to Study Neuroblast Migration In vitro

11,244 Views

•

11:32 min

•

November 12, 2013

DOI:

10.3791/50989-v

DOI:
10.3791/50989-v

•

11:32 min
November 12, 2013

•

1 Views
, , , ,
1Wolfson Centre for Age-Related Diseases,King’s College London, 2MRC Centre for Developmental Neurobiology,King’s College London

概要

Automatically generated

Neuroblast migration is a crucial step in postnatal neurogenesis. The protocol described here can be used to investigate the role of candidate regulators of neuroblast migration by employing DNA/small hairpin RNA (shRNA) nucleofection and a 3D migration assay with neuroblasts isolated from the rodent postnatal rostral migratory stream.

Explore More Videos

Subventricular ZoneNeuroblast MigrationRostral Migratory StreamOlfactory BulbNeurogenesisNucleofection3D In Vitro Migration AssayPlasmid DNAShRNASiRNAReaggregationSignaling Pathways

Read Article

Cite this Article

Falenta, K., Gajendra, S., Sonego, M., Doherty, P., Lalli, G. Nucleofection of Rodent Neuroblasts to Study Neuroblast Migration In vitro. J. Vis. Exp. (81), e50989, doi:10.3791/50989 (2013).

Download .ris file

Copy

Share Video

.

Copy