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Nucleofection of Rodent Neuroblasts to Study Neuroblast Migration In vitro
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Neuroscience
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JoVE Journal Neuroscience
Nucleofection of Rodent Neuroblasts to Study Neuroblast Migration In vitro

Nucleofection of Rodent Neuroblasts to Study Neuroblast Migration In vitro

English

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11,234 Views

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11:32 min

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November 12, 2013

DOI:

10.3791/50989-v

DOI:
10.3791/50989-v

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11:32 min
November 12, 2013

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1 Views
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1Wolfson Centre for Age-Related Diseases,King’s College London, 2MRC Centre for Developmental Neurobiology,King’s College London

Summary

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Neuroblast migration is a crucial step in postnatal neurogenesis. The protocol described here can be used to investigate the role of candidate regulators of neuroblast migration by employing DNA/small hairpin RNA (shRNA) nucleofection and a 3D migration assay with neuroblasts isolated from the rodent postnatal rostral migratory stream.

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Cite this Article

Falenta, K., Gajendra, S., Sonego, M., Doherty, P., Lalli, G. Nucleofection of Rodent Neuroblasts to Study Neuroblast Migration In vitro. J. Vis. Exp. (81), e50989, doi:10.3791/50989 (2013).

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