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Nucleofection of Rodent Neuroblasts to Study Neuroblast Migration In vitro
JoVE 杂志
神经科学
This content is Free Access.
JoVE 杂志 神经科学
Nucleofection of Rodent Neuroblasts to Study Neuroblast Migration In vitro

Nucleofection of Rodent Neuroblasts to Study Neuroblast Migration In vitro

DOI:
10.3791/50989-v

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11:32 min

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November 12, 2013

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, , , ,
1Wolfson Centre for Age-Related Diseases,King’s College London, 2MRC Centre for Developmental Neurobiology,King’s College London

Chapters

  • 00:05Title
  • 01:19Dissection and Dissociation of Rat RMS Neuroblasts
  • 04:30Nucleofection
  • 06:43Embedding
  • 08:50Results: Nucleofection of Rodent Neuroblasts to Study Neuroblast Migration In vitro
  • 11:09Conclusion

Summary

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Neuroblast migration is a crucial step in postnatal neurogenesis. The protocol described here can be used to investigate the role of candidate regulators of neuroblast migration by employing DNA/small hairpin RNA (shRNA) nucleofection and a 3D migration assay with neuroblasts isolated from the rodent postnatal rostral migratory stream.

Tags

Keywords: Subventricular ZoneNeuroblast MigrationRostral Migratory StreamOlfactory BulbNeurogenesisNucleofection3D In Vitro Migration AssayPlasmid DNAShRNASiRNAReaggregationSignaling Pathways

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