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培養された膀胱上皮を越えるヒト好中球の移行の定量的評価
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Immunology and Infection
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JoVE Journal Immunology and Infection
Quantitative Assessment of Human Neutrophil Migration Across a Cultured Bladder Epithelium
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培養された膀胱上皮を越えるヒト好中球の移行の定量的評価

日本語

Automatically Generated

14,903 Views

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11:20 min

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November 07, 2013

DOI:

10.3791/50919-v

DOI:
10.3791/50919-v

•

11:20 min
November 07, 2013

•

1 Views
,
1Department of Pediatrics,Washington University School of Medicine, 2Department of Molecular Microbiology,Washington University School of Medicine

Summary

Automatically generated

私たちは、尿路病原性大腸菌で膀胱の感染中の急性の炎症反応の重要な要素を模倣したin vitroモデルを開発しました。 transuroepithelial好中球遊走アッセイは、細菌感染や化学誘引物質に反応して、透過性の支持体上で培養、膀胱上皮間でヒト好中球の遊走の定量的評価が可能になります。

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Cite this Article

Lau, M. E., Hunstad, D. A. Quantitative Assessment of Human Neutrophil Migration Across a Cultured Bladder Epithelium. J. Vis. Exp. (81), e50919, doi:10.3791/50919 (2013).

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