/
/
Quantitative Assessment of Human Neutrophil Migration Across a Cultured Bladder Epithelium
Se requiere una suscripción a JoVE para ver este contenido. Inicie sesión o comience su prueba gratuita.
Quantitative Assessment of Human Neutrophil Migration Across a Cultured Bladder Epithelium
14,903 Views
•
11:20 min
•
November 07, 2013
DOI:
Summary
Automatically generated
We developed an in vitro model that mimics an important component of the acute inflammatory response during infection of the bladder with uropathogenic Escherichia coli. The transuroepithelial neutrophil migration assay enables quantitative assessment of human neutrophil migration across bladder epithelia, cultured on permeable supports, in response to bacterial infection or chemoattractant substances.
Read Article
Cite this Article
Lau, M. E., Hunstad, D. A. Quantitative Assessment of Human Neutrophil Migration Across a Cultured Bladder Epithelium. J. Vis. Exp. (81), e50919, doi:10.3791/50919 (2013).
Copy