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Quantitative Assessment of Human Neutrophil Migration Across a Cultured Bladder Epithelium
JoVE Journal
Imunologia e Infecção
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JoVE Journal Imunologia e Infecção
Quantitative Assessment of Human Neutrophil Migration Across a Cultured Bladder Epithelium

Quantitative Assessment of Human Neutrophil Migration Across a Cultured Bladder Epithelium

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11:20 min

November 07, 2013

DOI:

11:20 min
November 07, 2013

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Summary

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We developed an in vitro model that mimics an important component of the acute inflammatory response during infection of the bladder with uropathogenic Escherichia coli. The transuroepithelial neutrophil migration assay enables quantitative assessment of human neutrophil migration across bladder epithelia, cultured on permeable supports, in response to bacterial infection or chemoattractant substances.

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