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Quantitative Assessment of Human Neutrophil Migration Across a Cultured Bladder Epithelium
Chapters
- 00:05Title
- 01:27Seeding and Growing 5637 Cells on Permeable Supports |
- 03:46Isolation of Human Neutrophils from Peripheral Blood
- 06:22Transuroepithelial Neutrophil Migration Assay
- 09:56Results: PMN Migration Across Bladder Epithelia in Response to Various Stimuli
- 10:59Conclusion
We developed an in vitro model that mimics an important component of the acute inflammatory response during infection of the bladder with uropathogenic Escherichia coli. The transuroepithelial neutrophil migration assay enables quantitative assessment of human neutrophil migration across bladder epithelia, cultured on permeable supports, in response to bacterial infection or chemoattractant substances.
