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Quantitative Assessment of Human Neutrophil Migration Across a Cultured Bladder Epithelium
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JoVE 杂志 免疫与感染
Quantitative Assessment of Human Neutrophil Migration Across a Cultured Bladder Epithelium

Quantitative Assessment of Human Neutrophil Migration Across a Cultured Bladder Epithelium

14,903 Views

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11:20 min

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November 07, 2013

DOI:

10.3791/50919-v

DOI:
10.3791/50919-v

•

11:20 min
November 07, 2013

•

1 Views
,
1Department of Pediatrics,Washington University School of Medicine, 2Department of Molecular Microbiology,Washington University School of Medicine

Summary

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We developed an in vitro model that mimics an important component of the acute inflammatory response during infection of the bladder with uropathogenic Escherichia coli. The transuroepithelial neutrophil migration assay enables quantitative assessment of human neutrophil migration across bladder epithelia, cultured on permeable supports, in response to bacterial infection or chemoattractant substances.

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Cite this Article

Lau, M. E., Hunstad, D. A. Quantitative Assessment of Human Neutrophil Migration Across a Cultured Bladder Epithelium. J. Vis. Exp. (81), e50919, doi:10.3791/50919 (2013).

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