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Totale Estrazione di proteine ​​e 2-D elettroforesi su gel Metodi per<em> Burkholderia</em> Specie
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Immunology and Infection
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JoVE Journal Immunology and Infection
Total Protein Extraction and 2-D Gel Electrophoresis Methods for Burkholderia Species
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Totale Estrazione di proteine ​​e 2-D elettroforesi su gel Metodi per<em> Burkholderia</em> Specie

italiano

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28,793 Views

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08:31 min

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October 15, 2013

DOI:

10.3791/50730-v

DOI:
10.3791/50730-v

•

08:31 min
October 15, 2013

•

8 Views
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1Department of Pediatrics, Centre for Understanding and Preventing Infection in Children,University of British Columbia

Summary

Automatically generated

I membri del genere Burkholderia sono patogeni di importanza clinica. Noi descriviamo un metodo per l'estrazione delle proteine ​​totali batterica, utilizzando distruzione meccanica, e elettroforesi su gel 2-D per la successiva analisi proteomica.

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Cite this Article

Velapatiño, B., Zlosnik, J. E. A., Hird, T. J., Speert, D. P. Total Protein Extraction and 2-D Gel Electrophoresis Methods for Burkholderia Species. J. Vis. Exp. (80), e50730, doi:10.3791/50730 (2013).

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