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Cre Recombinase Kokain Klima yeri tercihi Hedef Genlerin Rolü Eğitim için ifade Viral Vektörler stereotaksik Mikroenjeksiyon
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Behavior
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JoVE Journal Behavior
Stereotaxic Microinjection of Viral Vectors Expressing Cre Recombinase to Study the Role of Target Genes in Cocaine Conditioned Place Preference
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Cre Recombinase Kokain Klima yeri tercihi Hedef Genlerin Rolü Eğitim için ifade Viral Vektörler stereotaksik Mikroenjeksiyon

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18,393 Views

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08:22 min

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July 30, 2013

DOI:

10.3791/50600-v

DOI:
10.3791/50600-v

•

08:22 min
July 30, 2013

•

2 Views
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1Graduate Program in Neuroscience,Weill Cornell Graduate School of Biomedical Sciences, 2Division of Pediatric Neurology, Department of Pediatrics,Weill Cornell Medical College

Summary

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Bu makalede, fare beynine viral vektörler microinject ve sonra satın alma, yok olma ve iade faz içeren klimalı yerde tercih paradigma test etmek için nasıl.

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Stereotaxic MicroinjectionViral VectorsCre RecombinaseConditional Gene KnockoutCocaineConditioned Place PreferenceAddictionBehavioral ModelsTemporally-specificRegionally-specificGene DeletionGene TargetingRAAVCocaine-induced PlasticityWithdrawalExtinctionReinstatement

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Schierberl, K. C., Rajadhyaksha, A. M. Stereotaxic Microinjection of Viral Vectors Expressing Cre Recombinase to Study the Role of Target Genes in Cocaine Conditioned Place Preference. J. Vis. Exp. (77), e50600, doi:10.3791/50600 (2013).

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