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Microiniezione stereotassica di vettori virali che esprimono Cre ricombinasi per studiare il ruolo di geni bersaglio in cocaina condizionata place preference
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Behavior
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JoVE Journal Behavior
Stereotaxic Microinjection of Viral Vectors Expressing Cre Recombinase to Study the Role of Target Genes in Cocaine Conditioned Place Preference
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Microiniezione stereotassica di vettori virali che esprimono Cre ricombinasi per studiare il ruolo di geni bersaglio in cocaina condizionata place preference

italiano

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18,393 Views

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08:22 min

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July 30, 2013

DOI:

10.3791/50600-v

DOI:
10.3791/50600-v

•

08:22 min
July 30, 2013

•

2 Views
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1Graduate Program in Neuroscience,Weill Cornell Graduate School of Biomedical Sciences, 2Division of Pediatric Neurology, Department of Pediatrics,Weill Cornell Medical College

Summary

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Questo articolo viene descritto come microinject vettori virali nel cervello di topo e poi prova in un luogo climatizzato preferenza paradigma che comprende una fase di acquisizione, l'estinzione e la reintegrazione.

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Stereotaxic MicroinjectionViral VectorsCre RecombinaseConditional Gene KnockoutCocaineConditioned Place PreferenceAddictionBehavioral ModelsTemporally-specificRegionally-specificGene DeletionGene TargetingRAAVCocaine-induced PlasticityWithdrawalExtinctionReinstatement

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Cite this Article

Schierberl, K. C., Rajadhyaksha, A. M. Stereotaxic Microinjection of Viral Vectors Expressing Cre Recombinase to Study the Role of Target Genes in Cocaine Conditioned Place Preference. J. Vis. Exp. (77), e50600, doi:10.3791/50600 (2013).

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