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Stereotaxic Microinjection of Viral Vectors Expressing Cre Recombinase to Study the Role of Target Genes in Cocaine Conditioned Place Preference
JoVE Journal
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JoVE Journal Davranış
Stereotaxic Microinjection of Viral Vectors Expressing Cre Recombinase to Study the Role of Target Genes in Cocaine Conditioned Place Preference

Stereotaxic Microinjection of Viral Vectors Expressing Cre Recombinase to Study the Role of Target Genes in Cocaine Conditioned Place Preference

DOI:
10.3791/50600-v

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08:22 min

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July 30, 2013

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,
1Graduate Program in Neuroscience,Weill Cornell Graduate School of Biomedical Sciences, 2Division of Pediatric Neurology, Department of Pediatrics,Weill Cornell Medical College

Bölümler

  • 00:05Başlık
  • 01:36Preparation and Setup for Stereotaxic Delivery of Viral Vectors
  • 02:32Stereotaxic Microinjection of Viral Vectors
  • 04:41Conditioned Place Reference: Acquisition, Extinction, and Reinstatement
  • 05:55Representative Cocaine Conditioned Place Preference Results
  • 07:39Conclusion

Özet

Otomatik Çeviri

Otomatik Çeviri

This article describes how to microinject viral vectors into mouse brain and then test in a conditioned place preference paradigm that includes an acquisition, extinction and reinstatement phase.

Etiketler

Stereotaxic MicroinjectionViral VectorsCre RecombinaseConditional Gene KnockoutCocaineConditioned Place PreferenceBağımlılıkBehavioral ModelsTemporally-specificRegionally-specificGene DeletionGene TargetingRAAVCocaine-induced PlasticityWithdrawalExtinctionReinstatement

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