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Stereotaxic Microinjection of Viral Vectors Expressing Cre Recombinase to Study the Role of Target Genes in Cocaine Conditioned Place Preference
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Stereotaxic Microinjection of Viral Vectors Expressing Cre Recombinase to Study the Role of Target Genes in Cocaine Conditioned Place Preference

Stereotaxic Microinjection of Viral Vectors Expressing Cre Recombinase to Study the Role of Target Genes in Cocaine Conditioned Place Preference

18,393 Views

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08:22 min

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July 30, 2013

DOI:

10.3791/50600-v

DOI:
10.3791/50600-v

•

08:22 min
July 30, 2013

•

2 Views
,
1Graduate Program in Neuroscience,Weill Cornell Graduate School of Biomedical Sciences, 2Division of Pediatric Neurology, Department of Pediatrics,Weill Cornell Medical College

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This article describes how to microinject viral vectors into mouse brain and then test in a conditioned place preference paradigm that includes an acquisition, extinction and reinstatement phase.

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Stereotaxic MicroinjectionViral VectorsCre RecombinaseConditional Gene KnockoutCocaineConditioned Place PreferenceAddictionBehavioral ModelsTemporally-specificRegionally-specificGene DeletionGene TargetingRAAVCocaine-induced PlasticityWithdrawalExtinctionReinstatement

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Schierberl, K. C., Rajadhyaksha, A. M. Stereotaxic Microinjection of Viral Vectors Expressing Cre Recombinase to Study the Role of Target Genes in Cocaine Conditioned Place Preference. J. Vis. Exp. (77), e50600, doi:10.3791/50600 (2013).

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