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La manipolazione genetica in<em> Δku80</em> Ceppi per analisi genomica funzionale di<em> Toxoplasma gondii</em
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Genetic Manipulation in Δku80 Strains for Functional Genomic Analysis of Toxoplasma gondii
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La manipolazione genetica in<em> Δku80</em> Ceppi per analisi genomica funzionale di<em> Toxoplasma gondii</em

italiano

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16,778 Views

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09:52 min

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July 12, 2013

DOI:

10.3791/50598-v

DOI:
10.3791/50598-v

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09:52 min
July 12, 2013

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6 Views
, , , , , ,
1Department of Microbiology and Immunology,The Geisel School of Medicine at Dartmouth

Summary

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Qui riportiamo un metodo per l'utilizzo di tipo I e tipo II<em> Δku80</em> Ceppi di<em> Toxoplasma gondii</em> Per generare in modo efficiente delezioni geniche mirate e sostituzioni del gene per l'analisi genomica funzionale.

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Cite this Article

Rommereim, L. M., Hortua Triana, M. A., Falla, A., Sanders, K. L., Guevara, R. B., Bzik, D. J., Fox, B. A. Genetic Manipulation in Δku80 Strains for Functional Genomic Analysis of Toxoplasma gondii. J. Vis. Exp. (77), e50598, doi:10.3791/50598 (2013).

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