Genetic Manipulation in <em>&Delta;ku80</em> Strains for Functional Genomic Analysis of <em>Toxoplasma gondii</em>

Leah M. Rommereim; Miryam A. Hortua Triana; Alejandra Falla; Kiah L. Sanders; Rebekah B. Guevara; David J. Bzik; Barbara A. Fox

doi:10.3791/50598

Journal

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Immunologie et infection

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Genetic Manipulation in Δku80 Strains for Functional Genomic Analysis of Toxoplasma gondii

Journal JoVE
Immunologie et infection

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Journal JoVE Immunologie et infection

Genetic Manipulation in Δku80 Strains for Functional Genomic Analysis of Toxoplasma gondii

Genetic Manipulation in Δku80 Strains for Functional Genomic Analysis of Toxoplasma gondii

16,785 Views

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09:52 min

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July 12, 2013

DOI:

10.3791/50598-v

DOI:

10.3791/50598-v

•

09:52 min

July 12, 2013

•

6 Views

Leah M. Rommereim¹, Miryam A. Hortua Triana¹, Alejandra Falla¹, Kiah L. Sanders¹, Rebekah B. Guevara¹, David J. Bzik¹, Barbara A. Fox¹

¹Department of Microbiology and Immunology,The Geisel School of Medicine at Dartmouth

Summary

Here we report a method for using type I and type II Δku80 strains of Toxoplasma gondii to efficiently generate targeted gene deletions and gene replacements for functional genomic analysis.

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Genetic Manipulation in Δku80 Strains for Functional Genomic Analysis of Toxoplasma gondii

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