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Genetic Manipulation in Δku80 Strains for Functional Genomic Analysis of Toxoplasma gondii
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Genetic Manipulation in Δku80 Strains for Functional Genomic Analysis of Toxoplasma gondii
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July 12, 2013
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Here we report a method for using type I and type II Δku80 strains of Toxoplasma gondii to efficiently generate targeted gene deletions and gene replacements for functional genomic analysis.
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Rommereim, L. M., Hortua Triana, M. A., Falla, A., Sanders, K. L., Guevara, R. B., Bzik, D. J., Fox, B. A. Genetic Manipulation in Δku80 Strains for Functional Genomic Analysis of Toxoplasma gondii. J. Vis. Exp. (77), e50598, doi:10.3791/50598 (2013).
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