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使用して樹状細胞の移行の監視<sup> 19</sup> F /<sup> 1</sup> H磁気共鳴イメージング
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Immunology and Infection
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JoVE Journal Immunology and Infection
Monitoring Dendritic Cell Migration using 19F / 1H Magnetic Resonance Imaging
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使用して樹状細胞の移行の監視<sup> 19</sup> F /<sup> 1</sup> H磁気共鳴イメージング

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11,697 Views

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08:12 min

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March 20, 2013

DOI:

10.3791/50251-v

DOI:
10.3791/50251-v

•

08:12 min
March 20, 2013

•

4 Views
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1Experimental and Clinical Research Center,A joint cooperation between the Charité Medical Faculty and the Max Delbrück Center for Molecular Medicine, 2Berlin Ultrahigh Field Facility (B.U.F.F.),Max Delbrück Center for Molecular Medicine

Summary

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MRIを用いて細胞の追跡は、過去数年間に顕著な注目を集めている。このプロトコルは、フッ素と樹状細胞のラベルを記述する（<sup> 19</sup> F）に富む粒子、これらの細胞のin vivoでの応用、で排出リンパ節への移動の程度を監視する<sup> 19</sup> F /<sup> 1</sup> H MRIと<sup> 19</sup> F MRS。

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Waiczies, H., Guenther, M., Skodowski, J., Lepore, S., Pohlmann, A., Niendorf, T., Waiczies, S. Monitoring Dendritic Cell Migration using 19F / 1H Magnetic Resonance Imaging. J. Vis. Exp. (73), e50251, doi:10.3791/50251 (2013).

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