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Monitoring Dendritic Cell Migration using 19F / 1H Magnetic Resonance Imaging
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JoVE Journal 免疫学と感染
Monitoring Dendritic Cell Migration using 19F / 1H Magnetic Resonance Imaging

Monitoring Dendritic Cell Migration using 19F / 1H Magnetic Resonance Imaging

DOI:
10.3791/50251-v

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08:12 min

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March 20, 2013

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, , , , , ,
1Experimental and Clinical Research Center,A joint cooperation between the Charité Medical Faculty and the Max Delbrück Center for Molecular Medicine, 2Berlin Ultrahigh Field Facility (B.U.F.F.),Max Delbrück Center for Molecular Medicine

章

  • 00:05標題
  • 01:46Labeling and In vivo Application of Dendritic Cells (DC) with 19F (Fluorine)-rich Particles
  • 02:47In vivo 19F/1H (Proton) Magnetic Resonance Imaging (MRI)
  • 05:04Lymph Node Magnetic Resonance Spectroscopy (MRS)
  • 06:20Results: Quantification of 19F-labeled DC Migration In vivo using 19F MRS
  • 07:33Conclusion

概要

自動翻訳

自動翻訳

Tracking of cells using MRI has gained remarkable attention in the past years. This protocol describes the labeling of dendritic cells with fluorine (19F)-rich particles, the in vivo application of these cells, and monitoring the extent of their migration to the draining lymph node with 19F/1H MRI and 19F MRS.

タグ

Magnetic Resonance ImagingMRICell TrackingDendritic CellsFluorine-19PerfluorocarbonCell LabelingCell MigrationIn Vivo ImagingContrast AgentsUSPIOT2 EnhancementBackground-free ImagingCell QuantificationMR Spectroscopy

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