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Monitoring Dendritic Cell Migration using 19F / 1H Magnetic Resonance Imaging
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Monitoring Dendritic Cell Migration using 19F / 1H Magnetic Resonance Imaging

Monitoring Dendritic Cell Migration using 19F / 1H Magnetic Resonance Imaging

11,696 Views

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08:12 min

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March 20, 2013

DOI:

10.3791/50251-v

DOI:
10.3791/50251-v

•

08:12 min
March 20, 2013

•

4 Views
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1Experimental and Clinical Research Center,A joint cooperation between the Charité Medical Faculty and the Max Delbrück Center for Molecular Medicine, 2Berlin Ultrahigh Field Facility (B.U.F.F.),Max Delbrück Center for Molecular Medicine

Summary

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Tracking of cells using MRI has gained remarkable attention in the past years. This protocol describes the labeling of dendritic cells with fluorine (19F)-rich particles, the in vivo application of these cells, and monitoring the extent of their migration to the draining lymph node with 19F/1H MRI and 19F MRS.

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Cite this Article

Waiczies, H., Guenther, M., Skodowski, J., Lepore, S., Pohlmann, A., Niendorf, T., Waiczies, S. Monitoring Dendritic Cell Migration using 19F / 1H Magnetic Resonance Imaging. J. Vis. Exp. (73), e50251, doi:10.3791/50251 (2013).

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