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Monitoraggio migrazione delle cellule dendritiche usando<sup> 19</sup> F /<sup> 1</sup> H Magnetic Resonance Imaging
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Immunology and Infection
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JoVE Journal Immunology and Infection
Monitoring Dendritic Cell Migration using 19F / 1H Magnetic Resonance Imaging
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Monitoraggio migrazione delle cellule dendritiche usando<sup> 19</sup> F /<sup> 1</sup> H Magnetic Resonance Imaging

italiano

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11,697 Views

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08:12 min

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March 20, 2013

DOI:

10.3791/50251-v

DOI:
10.3791/50251-v

•

08:12 min
March 20, 2013

•

4 Views
, , , , , ,
1Experimental and Clinical Research Center,A joint cooperation between the Charité Medical Faculty and the Max Delbrück Center for Molecular Medicine, 2Berlin Ultrahigh Field Facility (B.U.F.F.),Max Delbrück Center for Molecular Medicine

Summary

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Monitoraggio delle cellule utilizzando la risonanza magnetica ha guadagnato notevole attenzione negli ultimi anni. Questo protocollo descrive l'etichettatura delle cellule dendritiche con fluoro (<sup> 19</sup> Particelle F)-ricchi, l'applicazione in vivo di queste cellule, e monitorare l'entità della loro migrazione verso il linfonodo drenante con<sup> 19</sup> F /<sup> 1</sup> H MRI e<sup> 19</sup> F MRS.

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Cite this Article

Waiczies, H., Guenther, M., Skodowski, J., Lepore, S., Pohlmann, A., Niendorf, T., Waiczies, S. Monitoring Dendritic Cell Migration using 19F / 1H Magnetic Resonance Imaging. J. Vis. Exp. (73), e50251, doi:10.3791/50251 (2013).

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