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隔离和免疫淋巴细胞和树突状细胞的小鼠Peyer氏斑
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Immunology and Infection
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JoVE Journal Immunology and Infection
Isolating And Immunostaining Lymphocytes and Dendritic Cells from Murine Peyer’s Patches
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隔离和免疫淋巴细胞和树突状细胞的小鼠Peyer氏斑

DOI:
10.3791/50167-v

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09:49 min

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March 17, 2013

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, ,
1Division of Infectious Diseases,New York State Department of Health

Chapters

  • 00:05Title
  • 01:40Isolation of PP Cells for Flow Cytometry
  • 03:03Antibody Labeling of Cells for Flow Cytometry
  • 03:48Preparation of PP Cryosections
  • 04:49Cryosectioning
  • 05:20Antibody Labeling of Cryosections and Confocal Microscopy Analysis
  • 06:11Results: Immunological Analyses of Mouse Peyer’s Patches
  • 09:23Conclusion

Summary

Automatic Translation

Automatic Translation

理解在派伊尔氏淋巴集结的特定的细胞亚群（PPS），初级感应网站肠相关的淋巴组织的免疫功能有越来越大的兴趣。在这里，我们列出了流式细胞仪分析和PP冰冻切片免疫准备的PP单细胞准备的并行协议。

Tags

Peyer’s PatchesIntestinal ImmunosurveillanceM CellsFollicle-associated Epithelium (FAE)Dendritic Cells (DCs)MacrophagesLymphocytesFlow CytometryCryosectioningImmunostaining

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